DES alteration of StAR protein and P450c17 gene

Diethylstilbestrol inhibits the expression of the steroidogenic acute regulatory protein in mouse fetal testis

2004 Study Abstract

This study investigated the early deleterious effects of an in-utero exposure to diethylstilbestrol (DES) on mouse testicular development.

To that purpose, pregnant mice were injected daily with up to 100 microg/kg DES from 10.5 to 17.5 days postcoitum (dpc). At 18.5 dpc, testes were removed from fetuses for RNA (RT-PCR) and protein (Western blot, immunohistochemistry) analysis.

Twenty-two genes were selected among which transcription factors, markers of differentiation of the different testicular cell lineages, steroidogenic enzymes and hormone receptors.

The Steroidogenic Acute Regulatory (StAR) protein produced by the fetal Leydig cells was dramatically reduced in the DES-exposed testes.

The P450c17 was the other gene modified following DES exposure.

The alteration of these two genes is consistent with the decrease observed in the intratesticular testosterone levels, in the DES-exposed testes.

Collectively, we demonstrated that DES did not alter testicular cell lineage specification but that it strongly inhibited the major function of the fetal Leydig cells.

Sources

  • Diethylstilbestrol inhibits the expression of the steroidogenic acute regulatory protein in mouse fetal testis, Molecular and cellular endocrinology, NCBI PubMed PMID: 15196701, 2004 May 31.
  • Cells featured image by Henry de Valence.
DES DIETHYLSTILBESTROL RESOURCES

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