A genetic mechanism for DES-induced carcinogenesis ?

Use of representational difference analysis for the identification of mdm2 oncogene amplification in diethylstilbestrol-induced murine uterine adenocarcinomas

1994 Study Abstract

Exposure in utero to the synthetic estrogen diethylstilbestrol (DES) is associated with the subsequent development of reproductive-tract malignancies in female offspring.

To search for the genetic targets of DES, representational difference analysis was used to compare genomic DNA from DES-associated mouse uterine adenocarcinoma cells with genomic DNA from normal CD-1 mouse tissue.

Several difference clones were obtained, all of which recognized rearranged and amplified sequences in tumor compared with normal DNA.

  • One of these difference fragments mapped to a region of mouse chromosome 10 that includes the mdm2 oncogene.
  • Amplification and overexpression of mdm2 was found in all three early-passage cell lines established from independent DES-associated cancers.

These findings demonstrate the potential power of representational difference analysis in cancer research and suggest a genetic mechanism for DES-induced carcinogenesis.

  • Use of representational difference analysis for the identification of mdm2 oncogene amplification in diethylstilbestrol-induced murine uterine adenocarcinomas, Molecular carcinogenesis, NCBI PubMed PMID : 7916985, 1994 September.
  • Featured image credit researchgate.
DES DIETHYLSTILBESTROL RESOURCES

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